Biochemical Analysis of Spontaneous fepA Mutants of Escherichia coli
نویسندگان
چکیده
منابع مشابه
Escherichia coli and analysis of mutants
We have constructed a plasmid that, when introduced into Escherichia coil, induces the synthesis of large quantities of a protein with an apparent molecular mass of 66 kDa that differs from human immunodeficiency virus (HIV) RNA-dependent DNA polymerase (deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase or reverse transcriptase, EC 2.7.7.49) only in that it has two additional aminote...
متن کاملConfined Mobility of TonB and FepA in Escherichia coli Membranes
The important process of nutrient uptake in Escherichia coli, in many cases, involves transit of the nutrient through a class of beta-barrel proteins in the outer membrane known as TonB-dependent transporters (TBDTs) and requires interaction with the inner membrane protein TonB. Here we have imaged the mobility of the ferric enterobactin transporter FepA and TonB by tracking them in the membran...
متن کاملExpression of tolC and organic solvent tolerance of Escherichia coli ciprofloxacin resistant mutants
AcrAB-TolC is a major efflux pump in Escherichia coli. It was reported that tolC is overexpressed and involves in improving the organic solvent tolerance level in Escherichia coli marR mutants that are resistant to several antibiotics, such as ciprofloxacin. Low and intermediate levels resistance did not improve organic solvent tolerance. Thus, in this descriptive-experimental study it was deci...
متن کاملMucoid mutants of Escherichia coli.
The WV strain of Escherichia coli has been used extensively in analyzing various biosynthetic pathways (Davis, 1952a) and also in studying drug resistance (Davis, 1952b). This strain is nonmucoid as ordinarily cultured on solid medium at 35 C. However, among mutants selected for various properties, i. e., auxotrophy, meiotrophy4 and drug resistance, a few were observed to be mucoid. An investig...
متن کاملExpression of tolC and organic solvent tolerance of Escherichia coli ciprofloxacin resistant mutants
AcrAB-TolC is a major efflux pump in Escherichia coli. It was reported that tolC is overexpressed and involves in improving the organic solvent tolerance level in Escherichia coli marR mutants that are resistant to several antibiotics, such as ciprofloxacin. Low and intermediate levels resistance did not improve organic solvent tolerance. Thus, in this descriptive-experimental study it was deci...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1988
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-134-5-1355